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CABRI:ICLC Accession_number ICLC AL09001 Cell_line_name BALB/3T3 clone A31 Brief_description Species: mouse, mus musculus; Tissue: embryo Description Species: mouse, mus musculus; Tissue: embryo Depositor Prof. S. Penco, DIMES, Cell cultures laboratory , University of Genoa, Italy Bibliographic_reference - Morphology fibroblast, grown as monolayer Culture_conditions continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA, 37C, 5% CO2 Viruses Search for viruses was not performed Release_conditions Cell line available for distribution. For non-commercial investigative use only Hazard - Freezing_medium Culture medium 45% + 50% FCS + 5% DMSO Sterility mycoplasma negative, HOECHST and PCR Further_bibliography J Biol Chem. 1996; 271(30):17927-31. [PMID: 8663535 ] Proc Natl Acad Sci U S A. 1996; 93(16):8508-11. [PMID: 8710900 ] Comments embryo, 14 to 17 day gestation.

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